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By Myrtle A. Davis

Univ. of Maryland, Baltimore. Discusses laser scanning cytometry for the research of apoptosis, tissue-based equipment of quantification for reports utilizing animal types, and useful assays and ELISA recommendations. DNLM: Apoptosis--physiology.

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Extra resources for Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification

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Et al. (1999) Immunocytochemical detection and mapping of a cytokeratin 18 neo- epitope exposed during early apoptosis. J. Pathol. 187(5), 567–572. 65. Morsi, H. , Leers, M. , Kabarity, H. , and Jager, W. (2000) Apoptosis, bcl-2 expression, and proliferation in benign and malignant endometrial epithelium: An approach using multiparameter flow cytometry. Gynecol Oncol. 77(1), 11–17. Apoptotic Cell Death 35 66. , Ramaekers, F. , and Reutelingsperger, C. P. (1996) A novel assay to measure loss of plasma membrane asymmetry during apoptosis of adherent cells in culture.

218(2), 314–319. 12. , and Traganos, F. (1997) Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry 27(1), 1–20. 13. Hotz, M. , and Darzynkiewicz, Z. (1994) Flow cytometric detection of apoptosis: comparison of the assays of in situ DNA degradation and chromatin changes. Cytometry 15(3), 237–244. 14. , and Darzynkiewicz, Z. (1993) The cell cycle related differences in susceptibility of HL-60 cells to apoptosis induced by various antitumor agents.

For each measured object, the following parameters are recorded by LSC: 1. Integrated fluorescence intensity within the integration contour, which can be adjusted to a desired width with respect to the threshold contour; 2. ” 3. The area within the integration contour. 4. The perimeter of the contour (in micrometers). 5. Integrated fluorescence intensity within the area of a torus of a desired width defined by the peripheral contour located around (outside) the primary integration contour. , over the rim of cytoplasm of desired width outside the nucleus).

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