By Myrtle A. Davis
Univ. of Maryland, Baltimore. Discusses laser scanning cytometry for the research of apoptosis, tissue-based equipment of quantification for reports utilizing animal types, and useful assays and ELISA recommendations. DNLM: Apoptosis--physiology.
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Extra resources for Apoptosis Methods in Pharmacology and Toxicology: Approaches to Measurement and Quantification
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For each measured object, the following parameters are recorded by LSC: 1. Integrated fluorescence intensity within the integration contour, which can be adjusted to a desired width with respect to the threshold contour; 2. ” 3. The area within the integration contour. 4. The perimeter of the contour (in micrometers). 5. Integrated fluorescence intensity within the area of a torus of a desired width defined by the peripheral contour located around (outside) the primary integration contour. , over the rim of cytoplasm of desired width outside the nucleus).